Gram Stain Interpretation
Gram Positive Cocci Clusters ...
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Gram Stain Interpretation

Gram Positive Cocci Clusters or tetrads Coagulase (+) Staphylococcus aureus

Gram Positive Cocci Clusters or tetrads Coagulase (–) Novobiocin sensitive Staphylococcus lugdunensis, S. epidermidis

Gram Positive Cocci, Clusters or tetrads, Coagulase (–), Novobiocin resistant, Staphylococcus saprophyticus

Gram Positive Cocci, Long chains > 6, α-hemolytic, Viridans group (optochin resistant)

Gram Positive Cocci, Long chains > 6, β-hemolytic, Streptococcus pyogenes (GAS)

Gram Positive Cocci, Pairs and short chains < 6, α-hemolytic (partial, green hemolysis), Streptococcus pneumoniae (optochin sensitive), Enterococci (GDS) (also γ-hemolytic), Gemella (facultative anaerobe, variable hemolysis)

Gram Positive Cocci, Pairs and short chains < 6, β-hemolytic (complete hemolysis), Streptococcus agalactiae (GBS)

Gram Positive Cocci, Pairs and short chains < 6, γ-hemolytic (no hemolysis) Streptococcus bovis (GDS, variable hemolysis)

Gram Positive Cocci, Anaerobic, γ-hemolytic, Peptostreptococcus

Gram Positive Rods, Large (and spore forming), Aerobic, Bacillus

Gram Positive Rods, Large (and spore forming), Anaerobic Clostridium

Gram Positive Rods, Short, Aerobe Gardnerella

Gram Positive Rods, Short, Facultative anaerobe Listeria, Erisypelothrix

Gram Positive Rods, Short, Anaerobe Lactobacillus

Gram Positive Rods, Irregular/Pleomorphic, Aerobe (“club” shaped), Corynebacterium

Gram Positive Rods, Irregular/Pleomorphic, Anaerobe, Propionibacterium

Gram Positive Rods, Filamentous, Aerobe, Nocardia, Tropheryma

Gram Positive Rods, Filamentous, Anaerobe Actinomyces

Gram Negative, Cocci, CSF or genital, Facultative intracellular, Neisseria

Gram Negative, Cocci, Lower respiratory, Aerobe, Moraxella

Gram Negative, Coccobacilli and Pleomorphic, Aerobic, Respiratory and Oropharyngeal, Acinetobacter, Bordetella, Haemophilus (H. parainfluenzae = HACEK), Cardiobacterium hominis (HACEK), Kingella kingae (HACEK)

Gram Negative, Coccobacilli and Pleomorphic, Aerobic, Zoonoses, Brucella, Francisella, Bartonella

Gram Negative, Coccobacilli and Pleomorphic, Facultative Anaerobe, Respiratory and Oropharyngeal, Aggregatibacter actinomycetemcomitans (HACEK)

Gram Negative, Coccobacilli and Pleomorphic, Anaerobic,  Eikenella corrodens (HACEK)

Gram Negative, Curved Rods, Microaerophilic Campylobacter, Helicobacter

Gram Negative, Curved Rods, Halophilic, Vibrio

Gram Negative, Straight Rods, Obligate Aerobes (all lactose non-fermenters), Oxidase Negative, Stenotrophomonas

Gram Negative, Straight Rods, Obligate Aerobes (all lactose non-fermenters), Oxidase Variable, Burkholderia

Gram Negative, Straight Rods, Obligate Aerobes (all lactose non-fermenters), Oxidase Positive, Pseudomonas, Alcaligenes

Gram Negative, Straight Rods, Aerobes, Respiratory, Legionella

Gram Negative, Straight Rods, Aerobes, Enteric Gram Negative Rods: Lactose Fermenting (Coliforms), Escherichia, Enterobacter, Klebsiella, Citrobacter, Serratia (slow fermenters)

Gram Negative, Straight Rods, Aerobes, Enteric Gram Negative Rods: Non-Lactose Fermenting, Proteus, Salmonella, Shigella

Gram Negative, Straight Rods, Aerobes, Zoonoses, Pasteurella, Yersinia

Gram Negative, Straight Rods, Anaerobes, Bacteroides, Fusobacterium, Prevotella



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